Recovery of recombinant proteins CFP10 and ESAT6 from Escherichia coli inclusion bodies for tuberculosis diagnosis: a statistical optimization approach
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چکیده
منابع مشابه
Optimizing Primary Recovery and Refolding of Human Interferon-b from Escherichia coli Inclusion Bodies
Background: The refolding of proteins from inclusion bodies is affected by several factors, including solubilization of inclusion bodies by denaturants, removal of the denaturant, and assistance of refolding by small molecule additives. Objectives: The purpose of this study was optimization of recombinant human interferon-b purification in order to achieve higher efficiency, yield, and a produ...
متن کاملoptimizing primary recovery and refolding of human interferon-b from escherichia coli inclusion bodies
background: the refolding of proteins from inclusion bodies is affected by several factors, including solubilization of inclusion bodies by denaturants, removal of the denaturant, and assistance of refolding by small molecule additives. objectives: the purpose of this study was optimization of recombinant human interferon-b purification in order to achieve higher efficiency, yield, and a produc...
متن کاملOral Immunogenicity of Plant-Made Mycobacterium tuberculosis ESAT6 and CFP10
Two lines of transgenic carrot plants producing Mycobacterium tuberculosis proteins (ESAT6 and CFP10) have been constructed. The target proteins are present in carrot storage roots at a level not less than 0.056% of the total storage protein (TSP) for ESAT6 and 0.002% of TSP for CFP10. As has been shown, oral immunization of mice induces both the cell-mediated and humoral immunities. These data...
متن کاملRecovery of soluble, active recombinant protein from inclusion bodies.
In summary, three conditions need to be fulfilled to successfully sequence directly on large DNA templates. First, the amount of DNA in the sequencing reaction must be in the range of 3–4 μg. In this way, more DNA molecules will be available for priming during the first cycles of the PCR. Second, the DNA must be highly pure because relatively large amounts of DNA must be added in the sequencing...
متن کاملTargeted expression, purification, and cleavage of fusion proteins from inclusion bodies in Escherichia coli.
Today, proteins are typically overexpressed using solubility-enhancing fusion tags that allow for affinity chromatographic purification and subsequent removal by site-specific protease cleavage. In this review, we present an alternative approach to protein production using fusion partners specifically designed to accumulate in insoluble inclusion bodies. The strategy is appropriate for the mass...
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ژورنال
عنوان ژورنال: Biotechnology Research and Innovation
سال: 2019
ISSN: 2452-0721
DOI: 10.1016/j.biori.2019.08.003